Regulatory

Part:BBa_K5102069

Designed by: Natalia Kuźmierkiewicz   Group: iGEM24_Munich   (2024-09-30)


Woodchuck posttranscriptional regulatory element (WPRE)

The WPRE (Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element) is a sequence derived from the woodchuck hepatitis virus, commonly used in gene expression systems to enhance the stability and translation of mRNA. When incorporated into plasmids or viral vectors, WPRE improves the nuclear export and cytoplasmic accumulation of transgene mRNA, leading to increased protein expression levels.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 518
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None